Therefore, we examined plasma catecholamine concentration and blood pressure in wild-type and pendrin-null mice under basal conditions and then after 5 and 20 min of immobilization stress. Within the adrenal medulla, pendrin localizes to both epinephrine- and norepinephrine-producing chromaffin cells. However, immunohistochemistry and qPCR of microdissected adrenal zones showed that pendrin was expressed in the adrenal medulla, rather than in cortex. Pendrin protein was detected in adrenal lysates from wild-type but not pendrin-null mice. Therefore, we examined pendrin expression in the adrenal gland using PCR, immunoblots, and immunohistochemistry. However, serum aldosterone does not significantly increase in pendrin-null mice, suggesting that pendrin regulates adrenal zona glomerulosa aldosterone production. With pendrin gene ablation, blood pressure and vascular volume fall, which increases plasma renin concentration. Pendrin ( Slc26a4) is a Cl −/HCO 3 − exchanger expressed in renal intercalated cells and mediates renal Cl − absorption.
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